ANALYSIS OF DIFFERENTIAL EXPRESSION OF CLAUDIN-1 IN RELATION TO VEGF IN HEPG2 AND CACO-2 CELLS USING FLOW CYTOMETRY

Abstract

Background: The sustained polarity of epithelial cells, differentiation, and cell signaling are
underpinned by Claudins, which are the principal proteins of tight junctions, essential for vital cell
to-cell adherence. Claudins play a significant role in both physiology and pathogenesis, as numerous
studies have depicted their involvement in these processes. These proteins have an impact on normal
body functions and disease development, including tumors. Due to their cell-specific expression,
Claudins have been associated with the development of various tumors, suggesting their potential use
as diagnostic or prognostic markers. It can be suggested that could serve to predict or diagnose the
development of possible cancers. The objective of this study was to investigate the expression of
Claudin-1 associated with VEGF in the liver and colon cancer cell lines namely HepG2 and Caco-2
respectively.
Materials and Methods: In-vitro study of Claudin-1 expression linked to VEGF in HepG2 and Caco
2 cell lines This study incorporates HepG2 and Caco-2 cell lines. Expression of Claudin-1 in HepG2
and Caco-2 cells was determined after Flow Cytometry analysis.
Results: Claudin-1 showed different expression patterns in HepG2 and Caco-2 cells underscoring its
significant involvement in tumorigenesis and tumor progression.
Conclusion: The study evaluates the relationship between Claudin-1 and VEGF in cancer. It
highlights Claudin-1 as a key indicator to identify and predict cancer. This might open doors to new
treatment approaches.