COMPARISON OF EFFECTS OF TWO VARIETIES OF ALLIUM SATIVUM (ALLIUM SATIVUM VAR CHINESE EXOTIC AND ALLIUM SATIVUM VAR LEHSUN GULABI) ON SERUM GLUTATHIONE PEROXIDASE IN ALBINO RATS

Abstract

  ABSTRACT:

Objective: Glutathione peroxidase (GPx) is a family of multiple isozymes that catalyze the reduction of H2O2 or organic hydro peroxides to water or corresponding alcohols using reduced glutathione (GSH) as an electron donor. GPx competes with catalases for H2O2 as a substrate to protect against mild oxidative stress. Different medicinal plants and their active ingredients possess the ability to prevent decrease in GPx in oxidative stress. The objective of this study was to compare the effects of two varieties of Allium sativum on an antioxidative biomarker, serum glutathione peroxidase in albino rats.

Subjects and methods: It was a randomized controlled trial (RCT)

This study was conducted at Physiology Department, Services Institute of Medical Sciences (SIMS), Lahore from August 2012 to February 2014.The study was carried out on 120 male albino rats. The rats were randomly divided into four groups of thirty each. Group A was given normal saline (control); group B was administered hepatotoxic dose of acetaminophen (negative control); group C was pretreated with Allium sativum Var Chinese exotic extract for 7 days before receiving hepatotoxic dose of acetaminophen (Experimental 1); and group D was pretreated with Allium sativum Var Lehsun Gulabi extract for 7 days before receiving hepatoprotective dose of acetaminophen (Experimental 2). Serum glutathione peroxidase levels in each group were measured from terminal blood sampling done 24 hours after acetaminophen administration after ether anesthesia.

Results:

Highly significant (p=0.000) less reduction in serum glutathione peroxidase were manifested in experimental group D pretreated with ethanolic extract of Allium sativum Var Lehsun Gulabi as compared to reduction in this parameter in experimental group C pretreated with ethanolic extract of Allium sativum Var Chinese exotic.

Conclusion:

Allium sativum Var Lehsun Gulabi has better antioxidative potential as compared to Allium Sativum Var Chinese exotic